Quattro Micro NaICsI Calibration Data:
The package comprises a compact benchtop, robust, Quattro Micro triple quadrupole instrument with manufacturer spec performance (see data):
Quattro Micro Features include:
- High-sensitivity full-scan MS & MS/MS with high-selectivity from true triple quadrupole precursor ion (PI) and neutral loss (NL) scans
- Multiple reaction monitoring for quantification
- Fast and easy identification, characterisation, and quantification of metabolites
- Multimode capability in a single run
- Automated instrument optimization and quantitative method development tools
- Dedicated application managers for your specialised analytical requirements
- Small footprint (390mm x 587 x 810mm) that sits easily on your benchtop
Compound identity confirmation can be achieved with MS/MS using this instrument in ion scan mode - product ions are generated and detected for a specified precursor ion. The precursor and product ions, along with their ion ratios, can be used to confirm the identity of a compound.
The Quattro Micro high performance analyser is completed with an optically coupled, low noise/high gain orthologonal detector that approaches 100% efficiency for the detection of single ions. In this proprietary system the conventional electron multiplier has been suspended by a photomultiplier with a 10 year lifetime - simultaneously maximising sensitivity and minimising the cost of ownership.
The Astmospheric Pressure Ionisation (API) source features patented Z-SPRAY inlet technology for LC-MS-MS. The dual orthoganol geometry of the Quattro Micro ZSPRAY enables prolonged LC-MS-MS anaylsis of samples in complex biological or environmental matrices. ZSPRAY can even tolerate involatile buffers - saving time and money in HPLC method redevelopment. ZSPRAY is the only API interface available with an in-source isolation valve allowing the sampling cone to be rapidly removed for routine cleaning without breaking the vacuum - maximising up time. The inlet probe is mounted vertically for maximum space saving.
Automated analysis of complex samples is enabled using Data Directed Analysis (DDA). In automated LC-DDA-MS analyses, the Quattro micro API Mass Analyzer continuously records (typically MS) spectra throughout a chromatographic run to dynamically identify candidates for MS/MS analysis. Ions shortlisted for MS/MS analysis can be compared with a list of known background ions (such as phthalates) to exclude redundant analyses. When a component of interest elutes, the analyzer switches from MS to MS/MS mode. Simultaneously, the system selects collision energy values for each targeted precursor ion and records a product ion spectrum. After the MS/MS analysis is completed, the system returns to the selected survey mode to hunt for the next candidate. The most commonly used survey mode is the full scan MS. Precursor ions that are selected and analyzed may be dynamically appended to the exclusion list to prevent duplicate analyses.
Specific classes of compounds can be detected with MS/MS using the precursor ion or neutral loss mode. By detecting a specific product ion (precursor ion mode) or charged fragments resulting from a neutral loss (neutral loss mode), you can classify a compound of interest. These modes are helpful for monitoring classes of compounds like glycosylated peptides, glucuronide conjugates and aliphatic hydrocarbons.
Quattro Micro Specification:
- Acquisition rates up to 5000 amu/sec
- 2040 amu upper mass limit.
- Verticaly mounted liquid inlet probes to minimise tube length and peak dispersion.
- Software-controlled, integrated syringe pump for easy calibration, tuning and sample infusion.
- Software-controlled divert valve/manual injection valve.
-Control and data acquisition with MassLynx™ 4.1 & QuanLynx Software.
- Edwards E2M28 Rotary Pump.
The Waters Alliance 2695 Separations Module consists of a quarternary pump, degasser and a autosampler, capable of injecting from 5 x 24 2ml vials.
The Alliance four solvent in-line degasser offers a high efficiency alternative to helium sparging for the removal of dissolved gases from HPLC mobile phases.
The 2487 detector is a 2 channel, tunable ultraviolet/visible (UV/Vis) detector that operates in single or dual wavelength mode, allowing spectrum scanning using a flow cell or a cuvette, providing RatioPlot and MaxPlot functions.
Single Wavelength Mode
Single wavelength is the default mode of operation for the 2487, supporting monitoring of a single wavelength from 190nm to 700nm, settable in 1nm increments on Channel A.
Dual Wavelength Mode
In dual wavelength mode, the detector monitors on Channel A and Channel B. The sampling frequency is reduced from 10Hz to 1Hz, limiting use of this mode to more standard chromatography, where peaks span at least 20 seconds to enable full characterisation of a peak.
* PC Provided with MassLynx software installed. Note: we are unable to transfer licences therefore software licence purchase may be required from the manufacturer.