Waters Micromass Quattro Micro with Alliance LC-MS/MS

Quattro Micro with Alliance LC-MS/MS from Waters Micromass

Ref: st3287


6 month On-Site Warranty (UK)


£ Please Call

This superb Waters Micromass Quattro Micro API Alliance LC-MS/MS system comes complete with Waters Quattro Micro, 2695 Alliance, 2487 UV/Vis detector and PC with MassLynx installed. Undoubtedly produces the best calibration and quantification results of any Quattro Micro seen in our lab. New calibration data generated June 2018 - see below.

Are you an academic organisation? This system qualifies for a University discount
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System Details

Download Calibration Data for Quattro MicroQuattro Micro NaICsI Calibration Data:

The package comprises a compact benchtop, robust, Quattro Micro triple quadrupole instrument with manufacturer spec performance (see data):

Quattro Micro Features include:
  - High-sensitivity full-scan MS & MS/MS with high-selectivity from true triple quadrupole precursor ion (PI) and neutral loss (NL) scans
  - Multiple reaction monitoring for quantification
  - Fast and easy identification,  characterisation, and quantification of metabolites
  - Multimode capability in a single run
  - Automated instrument optimization and quantitative method development tools
  - Dedicated application managers for your specialised analytical requirements
  - Small footprint (390mm x 587 x 810mm) that sits easily on your benchtop

Compound identity confirmation can be achieved with MS/MS using this instrument in ion scan mode - product ions are generated and detected for a specified precursor ion. The precursor and product ions, along with their ion ratios, can be used to confirm the identity of a compound.


The Quattro Micro high performance analyser is completed with an optically coupled, low noise/high gain orthologonal detector that approaches 100% efficiency for the detection of single ions. In this proprietary system the conventional electron multiplier has been suspended by a photomultiplier with a 10 year lifetime - simultaneously maximising sensitivity and minimising the cost of ownership. 


The Astmospheric Pressure Ionisation (API) source features patented Z-SPRAY inlet technology for LC-MS-MS. The dual orthoganol geometry of the Quattro Micro ZSPRAY enables prolonged LC-MS-MS anaylsis of samples in complex biological or environmental matrices. ZSPRAY can even tolerate involatile buffers - saving time and money in HPLC method redevelopment. ZSPRAY is the only API interface available with an in-source isolation valve allowing the sampling cone to be rapidly removed for routine cleaning without breaking the vacuum - maximising up time. The inlet probe is mounted vertically for maximum space saving.


Automated analysis of complex samples is enabled using Data Directed Analysis (DDA). In automated LC-DDA-MS analyses, the Quattro micro API Mass Analyzer continuously records (typically MS) spectra throughout a chromatographic run to dynamically identify candidates for MS/MS analysis. Ions shortlisted for MS/MS analysis can be compared with a list of known background ions (such as phthalates) to exclude redundant analyses. When a component of interest elutes, the analyzer switches from MS to MS/MS mode. Simultaneously, the system selects collision energy values for each targeted precursor ion and records a product ion spectrum. After the MS/MS analysis is completed, the system returns to the selected survey mode to hunt for the next candidate. The most commonly used survey mode is the full scan MS. Precursor ions that are selected and analyzed may be dynamically appended to the exclusion list to prevent duplicate analyses.


Specific classes of compounds can be detected with MS/MS using the precursor ion or neutral loss mode. By detecting a specific product ion (precursor ion mode) or charged fragments resulting from a neutral loss (neutral loss mode), you can classify a compound of interest. These modes are helpful for monitoring classes of compounds like glycosylated peptides, glucuronide conjugates and aliphatic hydrocarbons.

Quattro Micro Specification:
- Acquisition rates up to 5000 amu/sec
- 2040 amu upper mass limit.
- Verticaly mounted liquid inlet probes to minimise tube length and peak dispersion.
- Software-controlled, integrated syringe pump for easy calibration, tuning and sample infusion.
- Software-controlled divert valve/manual injection valve.
-Control and data acquisition with MassLynx™ 4.1 & QuanLynx Software.
- Edwards E2M28 Rotary Pump.   

2695 Alliance

The Waters Alliance 2695 Separations Module consists of a quarternary pump, degasser and a  autosampler, capable of injecting from 5 x 24 2ml vials.

The Alliance four solvent in-line degasser offers a high efficiency alternative to helium sparging for the removal of dissolved gases from HPLC mobile phases.

Alliance Solvent Management

Number of Solvents 1 to 4
Solvent conditioningVacuum degas, two operating modes, four chambers, < 5000µL internal volume per chamber 0.000 to 10.000 mL/min (0.050 to 5.00 mL/min typical) in 0.001 mL/min increments
Compressibility compensationAutomatic and continous
System delay volume < 650µL, independent of backpressure at 1mL/min
Plunger seal washIntegral, active, programmable 
Gradient profiles 11 gradient curves including linear, step (2), concave (4), and convex (4)  
Dry prime/wet primeAutomatic front panel control, SystemPrep function for automatic solvent(s) purge 
Flow ramping Time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rates
Maximum operating pressure5000 psi (345 bar) (0.010 to 3.000 mL/min) programmable upper and lower limits 
Composition range 0.0% to 100.0%, in 0.1% increments
Composition accuracy ±0.5% absolute, independent of backpressure (proportioning valve pair test, [degassed methanol: methanol/propylparaben, 2.0mL/min, 254nm]) 
Composition precision≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time (degassed methanol: water 60:40 dial-a-mix, 1.00mL/min, six replicates, phenone mix, 254nm) 
Flow precision≤0.075% RSD or ≤0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min), isocratic premix 
Flow rate range 0.000 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001mL/min increments 
Flow accuracy ±1% or 100µL/min whichever is greater, 0.200 to 5.000 mL/min (degassed methanol, at 600 psi backpressure) 

2487 UV/Vis Detector

The 2487 detector is a 2 channel, tunable ultraviolet/visible (UV/Vis) detector that operates in single or dual wavelength mode, allowing spectrum scanning using a flow cell or a cuvette, providing RatioPlot and MaxPlot functions.
Single Wavelength Mode
Single wavelength is the default mode of operation for the 2487, supporting monitoring of a single wavelength from 190nm to 700nm, settable in 1nm increments on Channel A.

Dual Wavelength Mode
In dual wavelength mode, the detector monitors on Channel A and Channel B. The sampling frequency is reduced from 10Hz to 1Hz, limiting use of this mode to more standard chromatography, where peaks span at least 20 seconds to enable full characterisation of a peak.


  • Wavelength range: 190nm to 700nm
  • Bandwidth: 5nm
  • Accuracy: ± 1nm
  • Repeatability: ±0.10nm
  • Sensitivity setting range: 0.0001 AUFS to 4.0000 AUFS
  • Lamp Source: Deuterium arc lamp
  • Flow cell: TaperSlit Flow Cell Design
  • Pressure limit: 1000psi / 70bar
  • Materials: 316 stainless steel, fused silica, Teflon.
  • Operating temperature: 4°C to 40°C
  • Operating humidity: 20% to 80%, noncondensing
  • Line frequency: 50Hz to 60Hz
  • Dimensions: 208mm(H) x 503mm (L) x 284mm (W)
  • Weight: 9.3 kg

Quattro Micro Alliance System Spec

Operating Voltage: 230 Volts AC.
Roughing Pump: Edwards E2M28
Software: MassLynx 4.1 / QuanLynx running on  PC with TFT
Printer:Samsung 191

* PC Provided with MassLynx software installed. Note: we are unable to transfer licences therefore software licence purchase may be required from the manufacturer.

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Servicing & Maintenance

As a continually expanding company Jaytee have a great deal to offer, from service agreements to total instrument portfolio management. Since each client has different requirements, we are able to provide tailored service agreements, whether it be on the instruments you purchase from us, or your own equipment.


Jaytee has developed its calibration policy in line with the marketplace and offers a wide range of calibration services. Our clients often require instrument PQ/validation, so we offer a unique approach to this, providing our own calibration package that is specific to your needs.

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Contact Information

Jaytee Biosciences Ltd,
Altira Business Park,
Herne Bay, Kent, CT6 6GZ.

Phone: +44 (0) 1227 265333

Email: Mailto sales jaytee

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Why employ ms4sale?

Your MS options are far reaching, so why use Jaytee Biosciences? Unlike other providers of used MS equipment, we have the ability to completely:

This is because we employ both skilled engineers and experienced scientists, which allows us to provide a value added solution.

When we acquire an MS system, we are not simply re-listing for sale with a mark-up. We service,test and run the system, producing chromatographic and mass spectral data before we advertise. When our clients ask us whether a particular system is suitable for their application, we provide detailed information, and offer a pre-visit service whereby you send us your sample, which we run and prove for your benefit. Why? Because we can, and because it helps you make the right choices.

Ask yourself why other companies are only showing stock images, with no data or proof of performance - because we have the skills, you get the peace of mind and the ongoing support for your method.

Finally, this is at no added cost, since our pricing policy is flexible, and therefore competitive - a win/win!

Jaytee; No take overs/mergers in over 35 years - just professional, solid, skilled on going support.