Waters Micromass ZQ 4000 LC Mass Spectrometer

ZQ 4000 LC/MS from Waters Micromass

Ref: st4435


6 month On-Site Warranty (UK)


£ 25,950

This refurbished ZQ 4000 system has been fully tuned and calibrated October 2016.
Single quadrupole mass spectrometer from Micromass with Alliance and 2487 UV. Capable of performing up to 32 MS experiments in a single chromatographic run.

See full tune and calibration data files for download.

Are you an academic organisation? This system applies for a University discount
Enquire for full details...

System Details

Quantification Data Waters ZQ2000
Quantification Data Waters ZQ2000

4000 LC-MS

  • Air cooled instrument
  • Mass Range: 4096 amu
  • es+, es- (APCI available - please enquire)
  • ESCi Multi-mode ionization enabled, allowing scanning on all 4 ionization modes simultaneosly
  • Small footprint (Width x Height x Depth): 390 mm x 587 mm x 810 mm (15.3 in x 23.1 in x 32.0 in)
  • Acquisition using a state-of-the-art Embedded Personal Computer Acquisition System (EPCAS™)
  • ZSpray™ dual orthogonal sampling interface provides unsurpassed sensitivity and ruggedness
  • Vertically mounted liquid inlet probes to minimize tube length and peak dispersion
  • Software-controlled, integrated syringe pump for easy calibration, tuning and sample infusion
  • Software-controlled divert valve/manual injection valve
  • Digital control of source gas flow rates
  • Differentially pumped vacuum system featuring singular air-cooled, split flow turbomolecular pump technology

2695 Alliance

The Waters 2695* Separations Module consists of a quarternary pump, degasser and a variable volume autosampler, capable of injecting from 5 x 24 2ml vials
*Note: Images display a 2690 badge - this unit has been upgraded to 2695 specification.

The four solvent in-line degasser offers a high efficiency alternative to helium sparging for the removal of dissolved gases from HPLC mobile phases.

Designed  for a wide  variety of applications, this reliable tool is a common work-horse in  most analytical and contract labs.  Built to meet labs rigorous requirements or routine analysis, suited to Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Featuring a large, easy-to-use LCD interface, take advantage of the quick autostart and  optimize your methods with a simple push of a button.  Manage up to 120 industry standard vials with the built in autosampler.


Solvent Management

Number of Solvents 1 to 4
Solvent conditioningVacuum degas, two operating modes, four chambers, < 5000µL internal volume per chamber 0.000 to 10.000 mL/min (0.050 to 5.00 mL/min typical) in 0.001 mL/min increments
Compressibility compensationAutomatic and continous
System delay volume < 650µL, independent of backpressure at 1mL/min
Plunger seal washIntegral, active, programmable 
Gradient profiles 11 gradient curves including linear, step (2), concave (4), and convex (4)  
Dry prime/wet primeAutomatic front panel control, SystemPrep function for automatic solvent(s) purge 
Flow ramping Time (0.01 to 30.00 min in 0.01 min increments) to reach maximum flow rates
Maximum operating pressure5000 psi (345 bar) (0.010 to 3.000 mL/min) programmable upper and lower limits 
Composition range 0.0% to 100.0%, in 0.1% increments
Composition accuracy ±0.5% absolute, independent of backpressure (proportioning valve pair test, [degassed methanol: methanol/propylparaben, 2.0mL/min, 254nm]) 
Composition precision≤0.15% RSD or ≤0.02 min SD, whichever is greater, based on retention time (degassed methanol: water 60:40 dial-a-mix, 1.00mL/min, six replicates, phenone mix, 254nm) 
Flow precision≤0.075% RSD or ≤0.02 min SD, six replicates, based on retention time or volumetric measures (0.200 to 5.000 mL/min), isocratic premix 
Flow rate range 0.000 to 10.000 mL/min (0.050 to 5.000 mL/min typical) in 0.001mL/min increments 
Flow accuracy ±1% or 100µL/min whichever is greater, 0.200 to 5.000 mL/min (degassed methanol, at 600 psi backpressure) 

2487 UV/Vis Detector

The 2487 detector is a 2 channel, tunable ultraviolet/visible (UV/Vis) detector that operates in single or dual wavelength mode, allowing spectrum scanning using a flow cell or a cuvette, providing RatioPlot and MaxPlot functions.
Single Wavelength Mode
Single wavelength is the default mode of operation for the 2487, supporting monitoring of a single wavelength from 190nm to 700nm, settable in 1nm increments on Channel A.

Dual Wavelength Mode
In dual wavelength mode, the detector monitors on Channel A and Channel B. The sampling frequency is reduced from 10Hz to 1Hz, limiting use of this mode to more standard chromatography, where peaks span at least 20 seconds to enable full characterisation of a peak.


  • Stand-alone programmability - Stores up to 10 user defined programs (or methods) consisting of up to 16 programmable timed  events and 2 threshold events each.
  • Integral erbium calibration reference - ensures wavelength accuracy
  • Automatic second order filter - Automatically enganged for wavelengths 370nm and greater and removed for wavelengths 369nm or less.
  • Spectrum scan and storage - supports spectrum scan, display, subtraction, storage and playback, in addition to standard tunable absorbance and UV/Vis functionality.
  • Cuvette qualification - facilities qualification of the detector by insertion of a standard in a cuvette without breaking any fluidic connections.
  • Cuvette sample analysis - allows the spectrum of any sample placed in the cuvette to be recorded.
  • Method editing and storage - supports basic method programming, storage and retrieval from the front panel
  • Full diagnostic capability - supports built in diagnostic tools to optimize functionality and performance.
  • Backward compatibility - operates as a 486 detector with Millenium Chomatography manager version 2.1n and lower, PowerLine, ExpertEase and other Waters products connected via the LAC/E interface using the IEEE-488 bus.


  • Wavelength range: 190nm to 700nm
  • Bandwidth: 5nm
  • Accuracy: ± 1nm
  • Repeatability: ±0.10nm
  • Sensitivity setting range: 0.0001 AUFS to 4.0000 AUFS
  • Lamp Source: Deuterium arc lamp
  • Flow cell: TaperSlit Flow Cell Design
  • Pressure limit: 1000psi / 70bar
  • Materials: 316 stainless steel, fused silica, Teflon.
  • Operating temperature: 4°C to 40°C
  • Operating humidity: 20% to 80%, noncondensing
  • Line frequency: 50Hz to 60Hz
  • Dimensions: 208mm(H) x 503mm (L) x 284mm (W)
  • Weight: 9.3 kg

Operating Voltage for the system: 230 Volts AC.

Roughing Pump: Edwards E2M28

System Includes

Software: MassLynx 4.1 running on PC running Windows 7 and TFT

Printer: Samsung 191

Cables & electronic manuals


* PC Provided with MassLynx software installed. Note: we are unable to transfer licences therefore software licence purchase may be required by the manufacturer.

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Servicing & Maintenance

As a continually expanding company Jaytee have a great deal to offer, from service agreements to total instrument portfolio management. Since each client has different requirements, we are able to provide tailored service agreements, whether it be on the instruments you purchase from us, or your own equipment.


Jaytee has developed its calibration policy in line with the marketplace and offers a wide range of calibration services. Our clients often require instrument PQ/validation, so we offer a unique approach to this, providing our own calibration package that is specific to your needs.

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Contact Information

Jaytee Biosciences Ltd,
Altira Business Park,
Herne Bay, Kent, CT6 6GZ.

Phone: +44 (0) 1227 265333

Email: Mailto sales jaytee

Why employ ms4sale?

Your MS options are far reaching, so why use Jaytee Biosciences? Unlike other providers of used MS equipment, we have the ability to completely:

This is because we employ both skilled engineers and experienced scientists, which allows us to provide a value added solution.

When we acquire an MS system, we are not simply re-listing for sale with a mark-up. We service,test and run the system, producing chromatographic and mass spectral data before we advertise. When our clients ask us whether a particular system is suitable for their application, we provide detailed information, and offer a pre-visit service whereby you send us your sample, which we run and prove for your benefit. Why? Because we can, and because it helps you make the right choices.

Ask yourself why other companies are only showing stock images, with no data or proof of performance - because we have the skills, you get the peace of mind and the ongoing support for your method.

Finally, this is at no added cost, since our pricing policy is flexible, and therefore competitive - a win/win!

Jaytee; No take overs/mergers in over 35 years - just professional, solid, skilled on going support.